Ad Find the criteria to consider when selecting the right cell line for your experiments. SiMa cells were selected from forty-two cell lines screened for BoNTA complex uptake.
Interactions Of Antibodies To The Gram Negative Gastric Bacterium Helicobacter Pylori With The Synaptic Calcium Sensor Synaptotagmin 5 Correlate To Impaired Vesicle Recycling In Sima Human Neuroblastoma Cells Springerlink
The present version dates from 14th October 2018.
. Human Homo sapiensCell type. Add 50 ml of phosphate buffered saline containing 1 mM EDTA 1 mM EGTA and 1 mgmL glucose solution to flask to detach cells. Seed out at ca.
A human cell line designated as SiHa has been established from cancerous tissues of the cervix uteri. Database of Cross-Contaminated or Misidentified Cell Lines compiled by the International Cell Line Authentication Committee ICLAC. Results Introduction of Stabilized VAMP2 into the SiMa Neuroblastoma Cell Line for BoNTB Detection.
The human neuroblastoma SiMa cell line has been described as having a high degree of differentiation and high capacity for synthesis of neurotransmitters 25. 520 genes with high or low copy number in SIMA relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset. Neutralizing antibodies prevent the toxin-induced cleavage of SNAP-25.
Five stable genes were found for the SiMa cell line that could be used for normalization for all of the treatments while four were found for the IMR-32 cell line. Add rinse solution to the cells in centrifuge tube in step 1. Highly variable about 34-100 hours.
Browse by species cell type and research application. See Materials and methods section. This database is updated regularly to include new instances of false cell lines as these become known.
Ad Find the criteria to consider when selecting the right cell line for your experiments. Symbol Name Standardized Value. Every step of the way a wide range of cell health products.
Here we have analyzed the alterations of SV2A SV2B and SV2C in two human neuroblastoma cell lines SiMa and IMR-32 after treatment with B-27 and N-2. Maintaining healthy cells is the key to experimental success and reproducible research results. For the SiMa cell line the addition of B27N2 Supplement produced the most alterations after the seven-day treatment and VIP treatment the least.
The presence documented at initiation of an unprecedented cluster of poor-prognosis cytogenetic markers together with a high degree of differentiation combine to make SiMa a novel and unique tool for investigating Nb. The assay is serotype specific for either BoNTA or BoNTE which both cleave unique sequences on SNAP-25 within SiMa cells. Here we describe an in vitro assay using the SiMa cell line Marini et al 1999 that has been engineered to carry a stabilized VAMP molecule to report the BoNTB activity via a luminescent reaction.
Cells may grow slowly initially forming non-adherent large cell aggregates. Detection of N-MYC in dmin was confirmed by FISH and from Southern blots of cellular DNA using a 10 kb-sized N-MYC -specific probe. 3-5 x 10 6 cells80cm 2 or 04 x 10 6 cellsml.
SiMa is the first Nb cell line for which the in vivo provenance of the major cytogenetic changes has been thus demonstrated. Observe cells under an inverted microscope until cell layer is dispersed usually within 5. To give you confidence in the health of your cells every step of the way weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell.
Example of cell line screening. Such cells have already been successfully used for development of a cell-based potency assay for a commercial product of BoNTA toxin BOTOX as reported by Allergan 26. Western blots were performed with an antibody to SNAP25 and the percent SNAP25 cleavage was calculated.
A Immunoblot showing expression of APEX2- and NanoLuc-VAMP2 constructs in SiMa cells with expected molecular weights of 45 kD and 30 kD. Differentiated cells were treated with 1 nM BoNTA for 6 h followed by 16 h incubation to allow for the cleavage of SNAP25. The studies with light and electron microscopy have revealed the definite epithelial characteristics of the SiHa cells.
The end point is simple immunodetection of cleaved SNAP-25 from cell. Split confluent culture 13 to 15 every 3-6 days using trypsinEDTA or by tapping the flask. We describe the establishment and characterization of a new neuroblastoma Nb cell line SiMa carrying the major recurrent chromosome changes associated with poor prognosis Nb including amplification of N-MYC by formation of double minutes dmin der1t117p35q12 and der22t1722q22p13 and loss of chromosome 11 documented.
At 37 C with 5 CO 2. The end point is simple immunodetection of cleaved SNAP-25 from cell lysates with antibodies detecting only the newly exposed sequence on SNAP-25. SNORA80B small nucleolar RNA HACA box 80B 32803 RAD51AP2.
Both constructs carry the HA tag as evidenced by immunoblotting. Search Term Species - choose -. The assay is serotype specific for either BoNTA or BoNTE which both cleave unique sequences on SNAP-25 within SiMa cells.
23 rows SIMA - Cell line. The assay is serotype specific for either BoNTA or BoNTE which both cleave unique sequences on SNAP-25 within SiMa cells. Generation of stable SiMa cell lines with VAMP2 fusions to peroxidase APEX2 and luciferase NanoLuc.
256 genes mutated in the SIMA cell line from the COSMIC Cell Line Gene Mutation Profiles dataset. ABCA8 ATP-binding cassette sub-family A ABC1 member 8 ABCC12 ATP-binding cassette sub. In this preliminary proof of concept study we report the development of a neutralization test using the neuroblastoma SiMa cell line.
Browse by species cell type and research application. The catecholamine synthesis of the neuroblastoma cell lines SK-N-SH and SiMa was measured by HPLC as described in Materials and Methods.
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